Two NADPH-dependent 2-ketogluconate reductases involved in 2-ketogluconate assimilation in <i>Gluconobacter</i> sp. strain CHM43.

ABSTRACT: Incomplete oxidation of glucose by Gluconobacter sp. strain CHM43 produces gluconic acid and then 2- or 5-ketogluconic acid. Although 2-keto-D-gluconate (2KG) is a valuable compound, it is sometimes consumed by Gluconobacter itself via an unknown metabolic pathway. We anticipated that 2KG reductase (2KGR) would be a key enzyme in 2KG metabolism. GLF_0478 and GLF_1777 were identified in the genome of strain CHM43, which encode proteins with 70% and 48% amino acid sequence identity, respectively, to the 2KGR of Gluconobacter oxydans strain 621H. Constructed mutant derivatives of strain CHM43 lacking GLF_0478, GLF_1777, or both were examined for their 2KG consumption ability. Strains ∆GLF_0478 and ∆GLF_1777 consumed 2KG like the p

SUBMITTER: Nakashima S 

PROVIDER: S-EPMC11837542 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature