BBSome deficiency in Lotmaria passim reveals divergent functions in trypanosomatid parasites.

ABSTRACT:

Background

The BBSome is an octameric protein complex crucial for ciliary transport, though it also participates in multiple other cellular processes. These diverse functions may result from the co-option of its ancestral roles. Studying the BBSome in flagellated protists can provide insights into these ancestral functions and their subsequent adaptations.

Methods

We examined the functions of the BBSome (LpBBS1 and LpBBS2) in Lotmaria passim, a monoxenous trypanosomatid parasite infecting honey bees. The phenotypes resulting from depletion of LpBBS1 using the auxin-inducible degron system and disruption of LpBBS2 were characterized.

Results

Parasites deficient in LpBBS2 are smaller and less motile compared with wild-type. Although intraflagellar transport of a marker

SUBMITTER: Yuan X 

PROVIDER: S-EPMC11837635 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature