Density and entropy of immune cells within the tumor microenvironment of primary tumors and matched brain metastases.

ABSTRACT:

Background

Tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) have increasingly been reported to impact the brain metastatic process of solid tumors. However, data on intra-individual differences between primary tumor and brain metastasis (BM), as well as their correlation with clinical outcome parameters, is scarce.

Methods

We retrospectively identified patients who received resection of the primary tumor and BM between 01/1990 and 10/2022. Density quantification of TAMs (CD68⁺, CD163⁺) and TILs (CD3⁺, CD8⁺, CD45RO⁺, FOXP3⁺) was performed by immunohistochemical staining of matched tumor tissue samples. Images were processed with QuPath software and heterogeneity of generated heatmap

SUBMITTER: Kleinberger M 

PROVIDER: S-EPMC11837646 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature