Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR.

ABSTRACT:

Background

Real-time quantitative PCR (RT-qPCR) is a widely used method to investigate gene expression in neuroscience studies. Accurate relative quantification of RT-qPCR requires the selection of reference genes that are stably expressed across the experimental conditions and tissues of interest. While RT-qPCR is often performed to investigate gene expression changes during neurodevelopment, few studies have examined the expression stability of commonly used reference genes in the developing mouse cortex.

Results

Here, we evaluated the stability of five housekeeping genes, Actb, Gapdh, B2m, Rpl13a, and Hprt, in cortical tissue from mice at embryonic day 15 to postnatal day 0 to identify optimal reference genes with stable expression during late corticogenesis. The expressi

SUBMITTER: Uppalapati A 

PROVIDER: S-EPMC11837712 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature