Noninvasive characterization of oocyte deformability in microconstrictions.

ABSTRACT: Oocytes naturally present mechanical defects that hinder their development after fertilization. Thus, in the context of assisted reproduction, oocyte selection based on their mechanical properties has great potential to improve the quality of the resulting embryos and the success rate of these procedures. However, using mechanical properties as a quantifiable selective criterion requires robust and nondestructive measurement tools. This study developed a constriction-based microfluidic device that monitors the deformation of mouse oocytes under controlled pressure. The device can distinguish mechanically aberrant oocyte groups from healthy control ones. On the basis of a mathematical model, we propose that deformability measurements infer both oocyte tension and elasticity, elasticity bein

SUBMITTER: Barbier L 

PROVIDER: S-EPMC11838009 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature