Deuterium labeling enables proteome wide turnover kinetics analysis in cell culture.

ABSTRACT: The half-life of proteins is tightly regulated and underlies many cellular processes. It remains unclear the extent to which proteins are dynamically synthesized and degraded in different cell types and cell states. We introduce an improved D₂O labeling workflow and apply it to examine the landscape of protein turnover in pluripotent and differentiating human induced pluripotent stem cells (hiPSC). The majority of hiPSC proteins show minimal turnover beyond cell doubling rates, but we also identify over 100 new fast-turnover proteins not previously described as short-lived. These include proteins that function in cell division and cell cycle checkpoints, that are enriched in APC/C and SPOP degrons, and that are depleted upon pluripotency exit. Differentiation rapidly shifts the

SUBMITTER: Alamillo L 

PROVIDER: S-EPMC11838351 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature