Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labeling.

ABSTRACT: Deep molecular phenotyping of cells at transcriptomic and proteomic levels is an essential first step to understanding cellular contributions to development, aging, injury, and disease. Since proteome and transcriptome level abundances only modestly correlate with each other, complementary profiling of both is needed. We report a novel method called simultaneous protein and RNA -omics (SPARO) to capture the cell type-specific transcriptome and proteome simultaneously from both in vitro and in vivo experimental model systems. This method leverages the ability of biotin ligase, TurboID, to biotinylate cytosolic proteins including ribosomal and RNA-binding proteins, which allows enrichment of biotinylated proteins for proteomics as well as protein-associated RNA for transcriptomics. We valida

SUBMITTER: Ramelow CC 

PROVIDER: S-EPMC11838394 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature