Directed Evolution of a Plant Immune Receptor for Broad Spectrum Effector Recognition.

ABSTRACT: Rapid development of immune receptors that protect crops from emerging pathogens is a critical challenge ^1,2. While novel immune receptors that recognize previously undetected pathogen effectors could provide protection against a wider range of pathogens, engineering such receptors has been constrained by the low throughput and speed of in planta testing. We established yeast surface display as a high throughput platform to recapitulate plant immune receptor-ligand interactions and evolve new binding capabilities. Using this directed evolution platform, we engineered the ligand binding domain of the rice immune receptor Pik-1 to recognize diverse effectors from the fast-evolving fungal pathogen Magnaporthe oryzae. Our approach yielded Pik-1 ligand binding domains w

SUBMITTER: Rim EY 

PROVIDER: S-EPMC11838462 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature