Widespread mono- and oligoadenylation direct small noncoding RNA maturation versus degradation fates.

ABSTRACT: Small non-coding RNAs (sncRNAs) are subject to 3' end trimming and tailing activities that impact maturation versus degradation decisions during biogenesis. To investigate the dynamics of human sncRNA 3' end processing at a global level we performed genome-wide 3' end sequencing of nascently-transcribed and steady-state sncRNAs. This revealed widespread post-transcriptional adenylation of nascent sncRNAs, which came in two distinct varieties. One is characterized by oligoadenylation, which is transient, promoted by TENT4A/4B polymerases, and most commonly observed on unstable snoRNAs that are not fully processed at their 3' ends. The other is characterized by monoadenylation, which is broadly catalyzed by TENT2 and, in contrast to oligoadenylation, stably accumulates at the 3'-end of sncRN

SUBMITTER: Ocheltree C 

PROVIDER: S-EPMC11838476 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature