Evaluation of sequencing reads at scale using rdeval.

ABSTRACT:

Motivation

Large sequencing data sets are produced and deposited into public archives at unprecedented rates. The availability of tools that can reliably and efficiently generate and store sequencing read summary statistics has become critical.

Results

As part of the effort by the Vertebrate Genomes Project (VGP) to generate high-quality reference genomes at scale, we sought to address the community need for efficient sequencing data evaluation by developing rdeval, a standalone tool to quickly compute and dynamically display sequencing read metrics. Rdeval can either run on the fly or store key sequence data metrics in read 'sketches', with dramatic compression gains. Statistics can then be efficiently recalled from sketches for additional processing. Rdeval can convert fa*

SUBMITTER: Formenti G 

PROVIDER: S-EPMC11838479 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature