Context specific ubiquitin modification of ribosomes regulates translation under oxidative stress.

ABSTRACT: Cellular exposure to stress is known to activate several translational control pathways through ribosome ubiquitination. However, how unique patterns of ribosome ubiquitination act at the site-specific level to drive distinct modes of translation regulation remains unclear. To further understand the complexity of these ubiquitin signals, we developed a new targeted proteomics approach to quantify site-specific ubiquitin modification across the ribosome. This method increased the sensitivity and throughput of current approaches and allowed us to systematically measure the ubiquitin status of 78 ribosome peptides and ubiquitin linkages in response to stress. Using this method, we were able to detect the ubiquitination of several ribosome sites even in steady-state conditions, and to show tha

SUBMITTER: Dougherty SE 

PROVIDER: S-EPMC11838502 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature