A Temporal and Spatial Atlas of Adaptive Immune Responses in the Lymph Node Following Viral Infection.

ABSTRACT: The spatial organization of adaptive immune cells within lymph nodes is critical for understanding immune responses during infection and disease. Here, we introduce AIR-SPACE, an integrative approach that combines high-resolution spatial transcriptomics with paired, high-fidelity long-read sequencing of T and B cell receptors. This method enables the simultaneous analysis of cellular transcriptomes and adaptive immune receptor (AIR) repertoires within their native spatial context. We applied AIR-SPACE to mouse popliteal lymph nodes at five distinct time points after Vaccinia virus footpad infection and constructed a comprehensive map of the developing adaptive immune response. Our analysis revealed heterogeneous activation niches, characterized by Interferon-gamma (IFN-γ) production, durin

SUBMITTER: Jiang S 

PROVIDER: S-EPMC11838507 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature