A ggplot-based single-gene viewer reveals insights into the translatome and other nucleotide-resolution omics data.

ABSTRACT: Seeing is believing. Visualizing Ribo-seq and other sequencing data within genes of interest is a powerful approach to studying gene expression, but its application is limited by a lack of robust tools. Here, we introduce ggRibo, a user-friendly R package for visualizing individual gene expression, integrating Ribo-seq, RNA-seq, and other genome-wide datasets with flexible scaling options. ggRibo visualizes 3-nucleotide periodicity, a hallmark of translating ribosomes, within a gene-structure context, including introns and untranslated regions, enabling the study of novel ORFs, translation of different isoforms, and mechanisms of translational regulation. ggRibo can plot multiple Ribo-seq/RNA-seq datasets from different conditions for comparison. It also contains funct

SUBMITTER: Wu HL 

PROVIDER: S-EPMC11838514 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature