CiFi: Accurate long-read chromatin conformation capture with low-input requirements.

ABSTRACT: By coupling chromatin conformation capture (3C) with PacBio HiFi long-read sequencing, we have developed a new method (CiFi) that enables analysis of genome interactions across repetitive genomic regions with low-input requirements. CiFi produces multiple interacting concatemer segments per read, facilitating genome assembly and scaffolding. Together, the approach enables genomic analysis of previously recalcitrant low-complexity loci, and of small organisms such as single insect individuals.

SUBMITTER: McGinty SP 

PROVIDER: S-EPMC11838532 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature