Gene regulatory logic of the interferon-β enhancer contains multiple selectively deployed modes of transcription factor synergy.

ABSTRACT: Type I interferon IFNβ is a key regulator of the immune response, and its dysregulated expression causes disease. The regulation of IFNβ promoter activity has been a touchpoint of mammalian gene control research since the discovery of functional synergy between two stimulus-responsive transcription factors (TFs) nuclear factor kappa B (NFκB) and interferon regulatory factors (IRF). However, subsequent gene knockout studies revealed that this synergy is condition-dependent such that either NFκB or IRF activation can be dispensable, leaving the precise regulatory logic of IFNβ transcription an open question. Here, we developed a series of quantitative enhancer states models of IFNβ expression control and evaluated them with stimulus-response data from TF knockouts. Our analysis confirmed tha

SUBMITTER: Schiffman A 

PROVIDER: S-EPMC11838565 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature