Post-transcriptional Modifications of the Large Ribosome Subunit Assembly Intermediates in <i>E. coli</i> Expressing Helicase-Inactive DbpA Variant.

ABSTRACT: RNA post-transcriptional modifications are ubiquitous across all organisms and serve as fundamental regulators of cellular homeostasis, growth, and stress adaptation. Techniques for the simultaneous detection of multiple RNA modifications in a high-throughput, single-nucleotide-resolution manner are largely absent in the field, and developing such techniques is of paramount importance. We used the Escherichia coli ribosome as a model system to develop novel techniques for RNA post-transcriptional modification detection, leveraging its extensive and diverse array of modifications. For modification detection, we performed reverse transcriptase reactions in the presence of Mn²⁺ and quantified the reverse transcriptase deletions and misincorporations at modification positions

SUBMITTER: Gracia Mazuca LA 

PROVIDER: S-EPMC11838604 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature