From Natural Microbe Screening to Sustained Chitinase Activity in Exogenous Hosts.

ABSTRACT: Genetic parts and hosts can be sourced from nature to realize new functions for synthetic biology or to improve performance in a particular application environment. Here, we proceed from the discovery and characterization of new parts to stable expression in new hosts with a particular focus on achieving sustained chitinase activity. Chitinase is a key enzyme for various industrial applications that require the breakdown of chitin, the second most abundant biopolymer on the earth. Diverse microbes exhibit chitinase activity, but for applications, the environmental conditions for optimal enzyme activity and microbe fitness must align with the application context. Achieving sustained chitinase activity under broad conditions in heterologous hosts has also proven difficult due to toxic side e

SUBMITTER: Chaulagain D 

PROVIDER: S-EPMC11838836 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature