EndoMAP.v1, a Structural Protein Complex Landscape of Human Endosomes.

ABSTRACT: Early/sorting endosomes are dynamic organelles that play key roles in proteome control by triaging plasma membrane proteins for either recycling or degradation in the lysosome^1,2,3. These events are coordinated by numerous transiently-associated regulatory complexes and integral membrane components that contribute to organelle identity during endosome maturation⁴. While a subset of the several hundred protein components and cargoes known to associate with endosomes have been studied at the biochemical and/or structural level, interaction partners and higher order molecular assemblies for many endosomal components remain unknown. Here, we combine cross-linking and native gel mass spectrometry^5-8 of purified early endosomes with AlphaFold^9,10 and c

SUBMITTER: Gonzalez-Lozano MA 

PROVIDER: S-EPMC11839024 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature