Proteomic Landscape of Pattern Triggered Immunity in the Arabidopsis Leaf Apoplast.

ABSTRACT: The apoplast is a critical interface in plant-pathogen interactions particularly in the context of pattern-triggered immunity (PTI), which is initiated by recognition of microbe-associated molecular patterns (PAMPs). Our study characterizes the proteomic profile of the Arabidopsis apoplast during PTI induced by flg22, a 22 amino acid bacterial flagellin epitope, to elucidate the output of PTI. Apoplastic washing fluid (AWF) was extracted with minimal cytoplasmic contamination for LC-MS/MS analysis. We observed consistent identification of PTI enriched and depleted peptides across replicates with limited correlation between total protein abundance and transcript abundance. We observed topological bias in peptide recovery of receptor-like kinases with peptides predominantly recovered from th

SUBMITTER: Chen HC 

PROVIDER: S-EPMC11839045 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature