Next-Generation Protein Sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6.

ABSTRACT: The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies-single-molecule protein sequencing and individual ion mass spectrometry-for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteoform levels. For single-molecule protein sequencing, we employ the recently released Platinum^® instrument. Next-Generation Protein Sequencing^™ (NGPS^™) on Platinum utilizes cycles of N-terminal amino acid recognizer binding and aminopeptidase cleavage to enable parallelized sequencing of single peptide molecules. We found that NGPS produces single amino acid coverag

SUBMITTER: Skinner KA 

PROVIDER: S-EPMC11839055 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature