Phosphatidylinositol 4,5-bisphosphate Impacts Extracellular Vesicle Shedding from <i>C. elegans</i> Ciliated Sensory Neurons.

ABSTRACT: Small secreted extracellular vesicles (EVs) mediate intercellular transport of bioactive macromolecules. How the membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂), which plays a critical role in many cellular processes, impacts EV biogenesis is unclear. The primary cilium, a sensory organelle protruding from most non-dividing cells, transmits signals by shedding EVs called ectosomes. Here, we altered ciliary PI(4,5)P₂ by manipulating expression of the type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K1) PPK-1 and deletion of the phosphoinositide 5-phosphatase (INPP5E) inpp-1, then determined the impact on release of EVs that carried cargos tagged with fluorescent proteins. We discovered that increasing PI(4,5)P₂ differentially aff

SUBMITTER: Elsayyid MW 

PROVIDER: S-EPMC11839067 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature