Curvature generation and engineering principles from <i>Shewanella oneidensis</i> multi-flagellin flagellum.

ABSTRACT: Motility driven by nanoscale flagella is vital to microbial survival and spread in fluid and structured environments. Absence of native flagellum structures, however, has limited our understanding of the mechanisms of microbial motility, hindering efforts to engineer microbe-based microbots for applications. Here, by cryogenic electron tomography (cryoET) and microscopy (cryoEM), we determined the structural basis of motility driven by the single flagellum anchored to one pole of Shewanella oneidensis MR-1 (S. oneidensis), an electrogenic bacterium commonly used in biotechnology. The structures of the curved flagellum, representing the conformation during motion, are captured, allowing delineation of molecular interactions among the subunits of its three components-filament,

SUBMITTER: Lou Q 

PROVIDER: S-EPMC11839096 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature