Early cell cycle genes in cortical organoid progenitors predict interindividual variability in infant brain growth trajectories.

ABSTRACT: Human induced pluripotent stem cell (iPSC) derived cortical organoids (hCOs) model neurogenesis on an individual's genetic background. The degree to which hCO phenotypes recapitulate the brain growth of the participants from which they were derived is not well established. We generated up to 3 iPSC clones from each of 18 participants in the Infant Brain Imaging Study, who have undergone longitudinal brain imaging during infancy. We identified consistent hCO morphology and cortical cell types across clones from the same participant. hCO cross-sectional area and production of cortical hem cells were associated with in vivo cortical growth rates. Cell cycle associated genes expression in early progenitors at the crux of fate decision trajectories were correlated with cortical growth ra

SUBMITTER: Glass MR 

PROVIDER: S-EPMC11839139 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature