Changes in Protein Metabolism and Early Development of Sarcopenia in Mice With Cholestatic Liver Disease.

ABSTRACT:

Background

Sarcopenia is a frequent complication of liver cirrhosis. Here, we chose a mouse model of cholestatic liver disease (CLD) to gain mechanistic insights into the development of sarcopenia from the earliest stages of chronic liver injury. Particular attention was paid to protein metabolism, metabolite profiles, and mediators of CLD-induced muscle wasting.

Methods

Male C57BL/6 J mice underwent bile duct ligation (BDL), sham surgery, or served as untreated controls. The observation phase lasted from the preoperative stage to postoperative day 14. Metabolic cage experiments were performed to determine the nitrogen balance (N-BAL), nitrogen metabolite profiles, and total energy expenditure (TEE) using doubly labelled water. The fractional protein synthesis rate (FPSR) wa

SUBMITTER: Agrifoglio O 

PROVIDER: S-EPMC11839266 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature