NPA7: A Dual Receptor Activating Peptide That Inhibits Cardiac Oxidative Stress.

ABSTRACT:

Background

Cardiomyocyte oxidative stress significantly contributes to the progression of hypertension-induced heart failure, highlighting the need for targeted therapies. We developed a novel peptide, NPA7, that coactivates the GC-A (guanylyl cyclase A)/cGMP and MasR (Mas receptor)/cAMP pathway. This study aimed to test NPA7's ability to inhibit oxidative stress by modulating the p62 (Sequestosome 1)-KEAP1 (Kelch-like ECH-associated protein 1)-NRF2 (nuclear factor erythroid 2-related factor 2) pathway in human cardiomyocytes (HCMs) and a rat model of hypertension.

Methods

Oxidative stress was induced in HCMs using H₂O₂ with phosphate-buffered saline or NPA7 treatment. Intracellular reactive oxygen species levels were assessed via dihydroethidium staini

SUBMITTER: Ma X 

PROVIDER: S-EPMC11839381 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature