Read Length Dominates Phylogenetic Placement Accuracy of Ancient DNA Reads.

ABSTRACT: A common problem when analyzing ancient DNA data is to identify the species that corresponds to the recovered analyzing ancient DNA sequence(s). The standard approach is to deploy sequence similarity-based tools, such as BLAST. However, as analyzing ancient DNA reads may frequently stem from unsampled taxa due to extinction, it is likely that there is no exact match in any database. As a consequence, these tools may not be able to accurately place such reads in a phylogenetic context. Phylogenetic placement is a technique where a read is placed onto a specific branch of a phylogenetic reference tree, which allows for a substantially finer resolution when identifying reads. Prior applications of phylogenetic placement have deployed only on data from extant sources. Therefore, it is unclear

SUBMITTER: Bettisworth B 

PROVIDER: S-EPMC11839404 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature