A map of the rubisco biochemical landscape.

ABSTRACT: Rubisco is the primary CO₂-fixing enzyme of the biosphere¹, yet it has slow kinetics². The roles of evolution and chemical mechanism in constraining its biochemical function remain debated^3,4. Engineering efforts aimed at adjusting the biochemical parameters of rubisco have largely failed⁵, although recent results indicate that the functional potential of rubisco has a wider scope than previously known⁶. Here we developed a massively parallel assay, using an engineered Escherichia coli⁷ in which enzyme activity is coupled to growth, to systematically map the sequence-function landscape of rubisco. Composite assay of more than 99% of single-amino acid mutants versus CO₂ concentration enabled inference of

SUBMITTER: Prywes N 

PROVIDER: S-EPMC11839469 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature