Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library building methods.

ABSTRACT: While DNA barcoding methods are an increasingly important tool in biological conservation, the resource requirements of constructing reference libraries frequently reduce their efficacy. One efficient way of sourcing taxonomically validated DNA for reference libraries is to use museum collections. However, DNA degradation intrinsic to historical museum specimens can, if not addressed in the wet lab, lead to low quality data generation and severely limit scientific output. Several DNA extraction and library build methods that are designed to work with degraded DNA have been developed, although the ability to implement these methods at scale and at low cost has yet to be formally addressed. Here, the performance of widely used DNA extraction and library build methods are compared using museu

SUBMITTER: Marsh WA 

PROVIDER: S-EPMC11839992 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature