Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols.

ABSTRACT: The search has been ongoing for safe and effective antimicrobial agents for control and prevention of oral biofilm associated with disease. Clinical trials for oral specific anti-bacterials are costly and often provide inconclusive results. The simple approach of ex vivo testing of these agents has not demonstrated utility, likely due to variability of effects observed even with a single donor. We show how shed oral biofilms, easily obtained from donor saliva, and tested under optimized conditions, respond reproducibly to anti-bacterial challenges measured by reductions in rRNA accumulation in susceptible taxa. Responses are in part donor specific, but many bacteria taxa were shown to be reproducibly susceptible over a group of donors. For two antibiotics, vancomycin and penicillin G teste

SUBMITTER: Adami GR 

PROVIDER: S-EPMC11840115 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature