Two reductases complete steroidal glycoalkaloids biosynthesis in potato.

ABSTRACT: Steroidal glycoalkaloids (SGAs) are specialized metabolites primarily produced by Solanaceae plants such as potatoes and tomatoes. Notably, α-solanine and α-chaconine are recognized as toxic substances in potatoes. While the biosynthetic pathways of SGAs are largely understood, the final steps of α-solanine and α-chaconine biosynthesis remained elusive. In this study, we discovered that two reductase-encoding genes, reductase for potato glycoalkaloid biosynthesis 1 (RPG1) and RPG2, complete SGA biosynthesis in potato. Knockout of both RPG1 and RPG2 in potato hairy roots halted α-solanine production, leading to the accumulation of zwittersolanine. We analyzed the catalytic function of recombinant enzymes and conducted structural determination of the reaction products by nuclear magnetic res

SUBMITTER: Akiyama R 

PROVIDER: S-EPMC11840414 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature