Pre-transplant T-cell clonal analysis identifies CD8⁺ donor reactive clones that contribute to kidney transplant rejection.

ABSTRACT:

Introduction

Responses to allogeneic human leukocyte antigen (HLA) molecules limit the survival of transplanted organs. The changes in T-cell alloreactivity that contribute to this process, however, are not fully understood. We defined a set of donor reactive T-cell clones (DRTC) with the goal to elucidate signatures of kidney allograft rejection.

Methods

DRTC were identified pretransplant using an anti-donor mixed lymphocyte reaction assay: CFSE-diluting CD4⁺ and CD8⁺ DRTC were flow-sorted, and the TCR sequences were identified using Adaptive Immunosequencing. DRTC were then tracked in post-transplant biopsies, blood, and urine samples in a cohort of kidney transplant recipients.

Results

In patients with an abnormal biopsy, the majority of CD8

SUBMITTER: Sanders JM 

PROVIDER: S-EPMC11840674 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature