3D bioprintable Mg²⁺-incorporated hydrogels tailored for regeneration of volumetric muscle loss.

ABSTRACT: Rationale: Current therapeutic approaches for volumetric muscle loss (VML) face challenges owing to limited graft availability and insufficient bioactivity. Three-dimensional (3D) bioprinting has become an alternative technology for fabricating native tissue-mimetic grafts, allowing for tailored structures and complex designs. Methods: We developed an Mg²⁺-incorporated bioink composed of thiolated gelatin (GtnSH) and maleimide-conjugated gelatin (GtnMI) decorated with magnesium peroxide (MgO₂), referred to as a GtnSH/GtnMI/MgO₂ bioink. We designed in situ crosslinking between GtnSH and GtnMI to prepare cytocompatible bioink for 3D bioprinting of muscle mimetics. Results: The incorporated MgO₂ particles provided oxygen

SUBMITTER: Kang MS 

PROVIDER: S-EPMC11840723 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature