Poly (I:C)-induced inflammation requires the activation of toll-like receptor 3/Ca²⁺/CaMKII/pannexin 1-dependent signaling.

ABSTRACT: Pannexin1 (Panx1) is a glycoprotein, ubiquitously expressed throughout vertebrate tissues. In the cell membrane, it forms non-selective hemichannels (Panx1 HCs) that allow the release of ATP. This extracellular ATP triggers purinergic signaling relevant to the immune responses to pathogens, including viruses. While the activity of Panx1 HCs is known to be elevated by some viruses, the underlying molecular mechanism remains elusive. Methods: In this study, we used Poly(I:C), a double-stranded RNA analog that constitutes a hallmark of viral infections. Peritoneal macrophages were obtained from wild-type and Panx1 knock-out mice. The mRNA levels of proinflammatory cytokines were quantified by RT-qPCR. We also evaluated hemichannel activity through dye uptake assays, whereas Ca^2+</

SUBMITTER: Salgado M 

PROVIDER: S-EPMC11840745 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature