LongReadSum: A fast and flexible quality control and signal summarization tool for long-read sequencing data.

ABSTRACT: While several well-established quality control (QC) tools exist for short-read sequencing data, there is a general paucity of computational tools that efficiently deliver comprehensive metrics across a wide range of long-read sequencing data formats, such as Oxford Nanopore (ONT) POD5, ONT FAST5, ONT basecall summary, Pacific Biosciences (PacBio) unaligned BAM, and Illumina Complete Long Read (ICLR) FASTQ file formats. In addition to nucleotide sequence information, some file formats such as POD5 contain raw signal information used for base calling, while other file formats such as aligned BAM contain alignments to a linear reference genome or transcriptome and may also contain base modification information. There is currently no single available QC tool capable of summarizing each of thes

SUBMITTER: Perdomo JE 

PROVIDER: S-EPMC11840941 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature