GlnA3<i>_Mt</i> is able to glutamylate spermine but it is not essential for the detoxification of spermine in <i>Mycobacterium tuberculosis</i>.

ABSTRACT: Mycobacterium tuberculosis is well adapted to survive and persist in the infected host, escaping the host's immune response. Since polyamines such as spermine, which are synthesized by infected macrophages, are able to inhibit the growth of M. tuberculosis, the pathogen needs strategies to cope with these toxic metabolites. The actinomycete Streptomyces coelicolor, a close relative of M. tuberculosis, makes use of a gamma-glutamylation pathway to functionally neutralize spermine. We therefore considered whether a similar pathway would be functional in M. tuberculosis. In the current study, we demonstrated that M. tuberculosis growth was inhibited by the polyamine spermine. Using in vitro enzymatic assays we determined that GlnA3_Mt

SUBMITTER: Krysenko S 

PROVIDER: S-EPMC11841054 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature