A novel polysaccharide in the envelope of S. aureus influences the septal secretion of preproteins with a YSIRK/GXXS motif.

ABSTRACT: Bacteria transport proteins across the plasma membrane to assemble their envelope, acquire nutrients, and establish appropriate interactions with their environment. The majority of these proteins are synthesized as precursors with a cleavable N-terminal signal sequence for recognition by the Sec machinery. In Staphylococcus aureus, a small subset of secreted precursors carries a YSIRK/GXXS motif. This motif provides a pre-translocation function by promoting the targeting of precursors to septal membranes, but the trans-acting factors that regulate such spatial distribution are not known. Here, we used immunofluorescence-microscopy to compare the spatial trafficking of Staphylococcal protein A (SpA), an abundant YSIRK/GXXS bearing precursor, between mutants of an arranged tran

SUBMITTER: Ibrahim AM 

PROVIDER: S-EPMC11841062 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature