Effect of a high-fat diet and iron overload on erythropoiesis in mice.

ABSTRACT:

Background

Insulin and iron availability stimulate and regulate erythropoiesis, respectively. The effects of hyperinsulinemia and/or iron overload on erythroid differentiation are unclear.

Methodology

Male C57Bl/6J wild-type (WT) mice were fed a high-fat diet (HFD) (to produce hyperinsulinemia) or a control diet (CD) for varying periods (4-24 weeks). Hepcidin knock-out (Hamp1 ^-/- ) mice (which are iron-overloaded) were fed CD or HFD for 24 weeks. Terminal erythroid differentiation (TED) in the bone marrow (BM) from these mice was analyzed by flow cytometry. Hematological parameters were estimated in peripheral blood.

Results

HFD-feeding of WT mice did not significantly affect erythroid precursors in the BM or hematological parameters. However,

SUBMITTER: Varghese J 

PROVIDER: S-EPMC11841077 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature