An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium.

ABSTRACT: An improved sporulation method for Pythium aphanidermatum on 10% V₈-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virule

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC11841104 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature