A rhamnose-rich O-antigen of <i>Paraburkholderia phymatum</i> MP20 is required for symbiosis with <i>Mimosa pudica</i>.

ABSTRACT: Paraburkholderia phymatum, a β-proteobacterium, forms a nitrogen-fixing symbiosis with many species of the large legume genus Mimosa as well as with common bean (Phaseolus vulgaris L.). Paraburkholderia are considered to have evolved nodulation independently from the well-studied α-proteobacteria symbionts of legumes. However, the detailed mechanisms important for β-rhizobia-legume symbiosis have not yet been determined. In this manuscript, we have sequenced the genome of P. phymatum MP20, a strain isolated from Mimosa pudica nodules, and utilized transposon mutagenesis to identify a mutant that showed delayed and ineffective nodulation of M. pudica. Further analysis revealed that the mutant strain produced an altered lipopolysaccharide lack

SUBMITTER: Welmillage SU 

PROVIDER: S-EPMC11841133 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature