A non-structural protein 1 substitution of dengue virus enhances viral replication by interfering with the antiviral signaling pathway.

ABSTRACT:

Background

The largest dengue virus 2 (DENV2) outbreak occurred in Taiwan in 2015, resulting in many fatalities. We therefore aim to identify crucial genetic variations which determine the virulence of the 2015 Taiwan outbreak strains.

Methods

We compared the 2015 Taiwan DENV2 sequences to the pre-2015 sequences. Reverse genetics (rg) viruses with substitutions were produced and the viral growth kinetics were investigated. We treated A549 cells with interferon (IFN) to determine the interferon-stimulated genes (ISGs) expression and STAT1 phosphorylation in the rg viral infection and plasmid transfection systems. IFN and pro-inflammatory cytokines levels were measured upon DENV infection using ELISA.

Results

The rgNS1-K272R mutant showed faster replication in IFN-I pro

SUBMITTER: Hee JR 

PROVIDER: S-EPMC11841148 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature