K27-linked RORγt ubiquitination by Nedd4 potentiates Th17-mediated autoimmunity.

ABSTRACT:

Background

The HECT E3 ubiquitin ligase Nedd4 has been shown to positively regulate T cell responses, but its role in T helper (Th) cell differentiation and autoimmunity is unknown. Th17 cells are believed to play a pivotal role in the development and pathogenesis of autoimmune diseases. Nevertheless, the regulation of RORγt activation during Th17 cell differentiation by TCR signaling is yet to be elucidated. These uncharted aspects inspire us to explore the potential role of Nedd4 in Th17-mediated autoimmunity.

Methods

We evaluated the impact of Nedd4 deficiency on mouse T cell development and differentiation using flow cytometry and siRNA transfection, and subsequently validated these findings in T cells from patients with multiple sclerosis (MS). Furthermore, we investiga

SUBMITTER: Zeng Q 

PROVIDER: S-EPMC11841259 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature