The role of lipid phase and temperature in proton barrier and proton migration on biological membranes.

ABSTRACT: Biological membranes play a major role in diffusing protons on their surfaces between transmembrane protein complexes. The retention of protons on the membrane's surface is commonly described by a membrane-associated proton barrier that determines the efficiency of protons escaping from surface to bulk, which correlates with the proton diffusion (PD) dimensionality at the membrane's surface. Here, we explore the role of the membrane's biophysical properties and its ability to accept a proton from a light-triggered proton donor situated on the membrane's surface and to support PD around the probe. By changing lipid composition and temperature, while going through the melting point of the membrane, we directly investigate the role of the membrane phase in PD. We show that the proton transfer

SUBMITTER: Ramanthrikkovil Variyam A 

PROVIDER: S-EPMC11841289 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature