KAT8 catalyzes the acetylation of SEPP1 at lysine 247/249 and modulates the activity of CD8⁺ T cells via LRP8 to promote anti-tumor immunity in pancreatic cancer.

ABSTRACT:

Background

Pancreatic cancer (PC) remains one of the most lethal malignancies with unfavorable prognosis globally. Bioinformatics analysis predicted that SEPP1 was low expressed in PC and related to tumor immune microenvironment, but its biological function was still unclear.

Methods

PC xenograft and liver metastasis mouse models, as well as PC cell-MDSCs co-culture system, were established for in vivo and in vitro studies, respectively. The expression and localization of key molecules were detected by qRT-PCR, western blot, immunohistochemistry and immunofluorescence. Flow cytometry was employed to assess the abundance of immune cells and cell apoptosis. The interactions among KAT8, SEPP1 and LRP8 were detected by co-IP. Cell viability, migration and invasion were monitored

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC11841300 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature