Endothelial-mesenchymal crosstalk drives osteogenic differentiation of human osteoblasts through Notch signaling.

ABSTRACT:

Background

Angiogenesis and osteogenesis are closely interrelated. The interaction between endothelial and bone-forming cells, such as osteoblasts, is crucial for normal bone development and repair. Juxtacrine and paracrine mechanisms play key roles in cell differentiation towards the osteogenic direction, assuming the direct effect of endothelium on osteogenic differentiation. However, the mechanisms of this interplay have yet to be thoroughly studied.

Methods

Isolated endothelial cells (EC) from human umbilical vein and human osteoblasts (OB) from the epiphysis of the femur or tibia were cultured in direct and indirect (separated by membrane) contact in vitro under the osteogenic differentiation conditions. Osteogenic differentiation was verified by RT-PCR, and alizarin re

SUBMITTER: Perepletchikova D 

PROVIDER: S-EPMC11841332 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature