A Rapid Molecular Detection Tool for Toxigenic M1UK  Streptococcus pyogenes.

ABSTRACT:

Background

The gradual replacement of the Streptococcus pyogenes M1global genotype by a newly emergent M1UK variant is a global public health threat warranting increased surveillance. M1UK differs from progenitor M1global genotype by 27 single-nucleotide polymorphisms and is characterized by increased speA superantigen expression in vitro.

Methods

An allele-specific real-time polymerase chain reaction assay was developed for the rapid detection of M1UK strains. The assay was used in combination with whole genome sequencing to determine emm (sub)type distribution for 51 invasive (n = 9) and noninvasive (n = 42) S pyogenes clinical isolates.

Results

Emm1 was the most prevalent S pyogenes emm serotype (n = 11) in this set of clinical isolates, with M1UK being the dominan

SUBMITTER: Brouwer S 

PROVIDER: S-EPMC11841628 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature