Accessible LED Lightbox for Light-induced Retinal Damage in Pigmented Mice.

ABSTRACT:

Purpose

To design and validate a cost-effective and titratable system capable of light-induced photoreceptor dysfunction and damage in pigmented mice.

Methods

Two commonly used mouse strains in vision research, C57BL/6J and 129SVE mice, were exposed individually to varying light intensities in custom-designed boxes. Visual function was assessed by ERG, which was conducted two days prior, one day, and one week after light exposure. For morphological evaluation of photoreceptor health, we stained retinal sections with hematoxylin and eosin followed by light microscopy. Photoreceptor nuclei were quantified at equidistant points across the entire retina.

Results

C57BL/6J and 129SVE mice exhibited a significant reduction in visual function 1 day after exposure to light at

SUBMITTER: Aliff HL 

PROVIDER: S-EPMC11841687 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature