Molecular Analysis of Human Respiratory Syncytial Virus Group B Strains Isolated in Kenya Before and During the Emergence of Pandemic Influenza A/H1N1.

ABSTRACT:

Background

We conducted a retrospective study to explore molecular insights into human respiratory syncytial virus (HRSV) group B strains among patients attending outpatient clinics at government medical facilities both prior and during the onset of Influenza A/H1N1/2009 pandemic outbreak.

Methods

We screened 2300 nasopharyngeal swabs using multiplex real time reverse transcriptase polymerase chain reaction. We amplified a segment of the first and second hypervariable regions, as well as the conserved portion of the third domain of the G-gene using HRSV-B specific primers, sequenced by Sanger di-deoxy chain termination method and thereafter analyzed the sequences.

Results

We characterized the circulating strains into three known genotypes: SAB4 (1.4%), BA7 (1.4%), and

SUBMITTER: Wangui J 

PROVIDER: S-EPMC11842092 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature