Methylation of phase II metabolites of endogenous anabolic androgenic steroids to improve analytical performance.

ABSTRACT: The study of intact phase II metabolites of endogenous anabolic androgenic steroids (EAAS) gives important information about metabolism and has the potential to improve the detection of doping with testosterone. For analysis with liquid chromatography-mass spectrometry (LC-MS), chemical derivatization at the steroid moiety is a technique to improve the positive ionization efficiency of glucuronidated/sulfated EAAS under collision-induced dissociation (CID) conditions. However, regarding the chromatographic performance, there are still challenges to address, for example, poor peak shape, which is mainly caused by nondefined adsorption in the chromatographic system. Here, we show a novel derivatization technique for the analysis of selected phase II metabolites of EAAS, where the acidic moie

SUBMITTER: Pfeffer S 

PROVIDER: S-EPMC11842169 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature