Puccinia striiformis Effector PNPi Suppresses TaIAA14 Expression to Inhibit Host Cell Death Response.

ABSTRACT: Fungal pathogens secrete effectors that suppress the hypersensitive response (HR) of the host, characterised by programmed cell death, facilitating colonisation. However, how effectors manipulate host cell death remains poorly understood. In this study, we discovered that the Puccinia striiformis effector PNPi (Puccinia NPR1 interactor) suppressed BAX-induced cell death in Nicotiana benthamiana. This virulence was mediated by the FtsN domain of PNPi, and an enhanced suppression effect was observed when Ser¹²⁹ was mutated into arginine. Further RNA-sequencing analysis revealed that auxin signalling was disturbed, with the auxin-responsive protein IAA29-like (NbIAA29) being downregulated during cell death suppression by PNPi. Exogenous application of auxins alleviated cell death s

SUBMITTER: Wang H 

PROVIDER: S-EPMC11842466 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature