Ancient DNA HLA typing reveals significant shifts in frequency in Europe since the Neolithic.

ABSTRACT: Computational HLA typing has surged as a cost-effective strategy to uncover questions regarding the evolution of the HLA system, enabling immunogenic characterization from ancient DNA (aDNA) data. Nevertheless, it remains to be seen whether these methods are suitable for analyzing aDNA generated without target-enrichment. To investigate this, we evaluated the performance of five HLA typing tools using present-day data with simulated profiles typical of aDNA, as well as from high-coverage aDNA genomes downsampled at different read depths. We found that characterization of Class I genes at the first field resolution is feasible at read depths as low as 2x, where it retains an accuracy of ≈ 80%. Next, we used this insight to characterize HLA evolution in Europe from 154 ancient genomes by det

SUBMITTER: Plascencia AG 

PROVIDER: S-EPMC11842861 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature